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Whitepaper of High Content Image Fusion

March 14, 2023, Guangzhou



High Content Image Fusion (HCIF) uses the most cutting-edge image processing algorithm and real-time fusion technology, aiming to break through the limitation of insufficient depth of field under high-magnification objective lens, and can obtain a larger depth of field when adjusting the focal length, so as to obtain a clearer than real-time single-frame image. It is a robust and competitive software function available in Windows software Pixit Pro and App for smart devices.


  • HCIF function supports LANOPTIK 12MP and higher resolution cameras.

  • HCIF function works very well when using biological microscope and metallurgical microscope. As the coarse and fine focus adjustment of stereo microscopes always come with a relatively large Z-axis offset, this is a great challenge to the algorithm of real-time fusion. Therefore, we recommend LANOPTIK cameras with rear-focusing knob to work with stereo microscopes and can also obtain perfect high-content images.

  • Cameras with rear-focusing mechanism are available in two versions: manual knob and motorized control. The version of motorized control can be operated by software or App. Effective stroke: 15mm.

  • The maximum depth of field depends on the parameters of the objective lens used.

  • The current algorithm cannot fully distinguish whether a object in the field of view is required for observation, and some actual impurities may also appear in the fused image.




In the video, The image on the left i is the real-time image under the microscope, and the image on the right is the real-time fusion image. Different from the traditional image stacking technology, HCIF not only achieves instant fusion, but also optimizes the image quality through intelligent algorithms. You only need to fine-tune the focus to get a fused image in real time.


White Paper of High-content Image Fusion 202303
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Whitepaper of High Content Image Fusion